bdnf  (Elabscience Biotechnology)

Journal: IBRO Neuroscience Reports

Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

doi: 10.1016/j.ibneur.2026.03.012

Figure Lengend Snippet: Experimental Timeline: Rats were treated with EE, RJ, and EE + RJ for 14 consecutive days. Stress was induced on days 8–14. Behavioral tests were conducted on days 15–18. On day 19, the animals were decapitated to assess serum corticosterone and hippocampal BDNF levels.

Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Pre-treatment with royal jelly, environmental enrichment, and their combination role in stress induced neurobehavioral deficits in male rats

doi: 10.1016/j.ibneur.2026.03.012

Figure Lengend Snippet: The effects of EE and RJ treatment on the hippocampal BDNF expression. The BDNF levels in the stressed group were significantly decreased than in the control group. EE and RJ treatment increased BDNF levels in the stressed rats. *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001 v.s. the Non-stressed control group, respectively; ^^ and ^^^ represent p < 0.01 and p < 0.001 v.s. the Non-treatment stressed group, respectively.

Article Snippet: The evaluation of serum corticosterone levels and hippocampal BDNF levels was conducted using an enzyme-linked immunosorbent assay (ELISA) with a commercially available ELISA kit (E20160505043, Hangzhou Eastbiopharm Co. Ltd., Zhejiang Province, China) and rat BDNF ELISA kits from Boster Biological Technology Co. with a sensitivity limit of 4 pg/ml following the instructions provided by the manufacturer.

Techniques: Expressing, Control

Journal: iScience

Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

doi: 10.1016/j.isci.2026.115059

Figure Lengend Snippet: Persistent hyperalgesia in the RPP model (A and B) Paw withdrawal latency (A) and 50% paw withdrawal threshold (B) of each rat ( n = 3–6). (C) Representative histological images of DRG from T10-S4 by H&E staining. Arrow, Schwann cell; ∗, nuclei; #, cytoplasm. Scale bars, 50 μm and 25 μm. (D and E) Representative IHC images (D) and quantification (E) of BDNF in the DRG from T10-S4. Brown DAB staining indicates BDNF immunopositivity. The staining intensity was semi-quantitatively scored as follows: (1) weak, (2) moderate, and (3) intense. Scale bars, 50 μm. ( n = 3 biological replicates per group). (F) Serum BDNF level on days 12 and 24 of RPP modeling ( n = 3). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, two-way ANOVA (A and B) or one-way ANOVA (E and F).

Article Snippet: Rat BDNF ELISA Kit , Elabscience , Cat# E-EL-R1235.

Techniques: Staining

Journal: iScience

Article Title: A pharmacological rat model of recurrent pelvic pain exhibiting hyperalgesia and depression-like behaviors

doi: 10.1016/j.isci.2026.115059

Figure Lengend Snippet: Comorbid depression-like behavior in the RPP model (A and B) Serum BDNF (A) and 5-HT (B) levels in the RPP model ( n = 6). (C and D) Sucrose preference ratio (C) and immobility time (D) in the RPP model ( n = 8). Data are presented as the mean ± SD; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, paired t test (A and B) or two-way ANOVA (C and D).

Article Snippet: Rat BDNF ELISA Kit , Elabscience , Cat# E-EL-R1235.

Techniques: